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. 2021 Apr 1;11:7326. doi: 10.1038/s41598-021-86578-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Life-history traits of wild type and DNMT3.1^−/− under nutrient rich conditions. (a) Body length measurements and fitted von Bertalanffy growth for 15 D. magna individuals of DNMT3.1^−/− and wild type. Red and black lines correspond to fitted curved for DNMT3.1^−/− and wild type, respectively, and boxplot of their body lengths at 3^rd clutch stage. (b) Boxplot of the 15 D. magna’s clutch sizes cultured individually in nutrient rich conditions. All boxplot whiskers extend to the highest and lowest values, and the boxes extended from quartile 1 to quartile 3, with the middle line showing the median. n.s. denote no significant differences by pairwise t-test. (c) Bar graph of the cumulative offspring produced from the 15 individuals at 3rd clutch. Error bars indicate the standard deviation of the mean. n.s. denote no significant difference by unpaired t-test.