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. 2021 Apr 1;7:8. doi: 10.1038/s41514-021-00058-7

Fig. 5. Administration of NR to aging mice increases osteoprogenitor number.

Fig. 5

a Representative flow cytometry analysis (left) and percentage (right) of Osx1-TdRFP+ cells in the bone marrow of 20-month-old Osx1-Cre;TdRFP mice treated without or with 12 mM NR for 8 months (n = 4 mice/group). b Representative images of Alizarin Red staining of bone marrow stromal cells, from mice described in Fig. 3, cultured under osteogenic conditions for 21 days. c Representative images (left) and quantification (right) of Oil Red O Staining in bone marrow stromal cells, from mice described in Fig. 3, cultured under adipogenic conditions for 10 days (triplicate cultures). d, e Representative images (right) and quantification (left) of TRAP-positive osteoclasts containing three or more nuclei in cultures of bone marrow macrophages with M-CSF and RANKL for 5 days, from mice described in Fig. 3 (quadruplicate cultures) (d); or from untreated 24-month-old C57BL/6 female mice in the presence of the indicated doses of NR (quadruplicate cultures) (e). Data represent mean ± SD; p values by two-tailed t-test (a, c, d) or one-way ANOVA (e).