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. 2021 Apr 1;12(4):344. doi: 10.1038/s41419-021-03639-2

Fig. 4. LMP1 increases radioresistance of NPC cells through BNIP3.

Fig. 4

A After transfection with shBNIP3 and control for 24, 48, and 72 h, respectively, the proliferation of different NPC cells was detected by MTS assay. B After transfection with shBNIP3 and control for 24 h, flow cytometric analysis was performed to observe cell apoptosis. C After transfection with shBNIP3 and control for 24 h, CM, HM, and KM cells were treated with IR at 0, 2, 4, and 6 Gy and cultured for 2 weeks. Then, fixation, staining, and colony quantification were gradually executed. Top: the representative images of hexamethylpararosaniline stained clonies. Bottom: the survival curves of CM, HM, and KM cells were fit to the data using a linear quadratic model of radiation sensitivity. Data are expressed as means ± S.D. of three experiments. *p < 0.05, **p < 0.01. D After transfection with GFP-LC3 for 24 h and with IR at 4 Gy, the expression of LC3 I/II in CM, HM, and KM cells by Western blots. UN untreated, CON negative control.