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. 2021 Apr 1;11:7336. doi: 10.1038/s41598-021-86343-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of the family Botourmiaviridae and selected related families based on an alignment of RdRP aa sequences generated using MAFFT v7.450 with Blosum62 substitution matrix. The evolutionary history was inferred by using the Maximum Likelihood method and Le_Gascuel_2008 model with 4 gamma categories (+ G + I). All positions with less than 95% site coverage were eliminated, i.e., fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position (partial deletion option). Evolutionary analyses were conducted in MEGA X with 1000 bootstrap repeats⁸⁶. The Armillaria viruses are designated with a star.