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. 2021 Apr 1;12(4):338. doi: 10.1038/s41419-021-03619-6

Fig. 3. UTD1 induced apoptosis in CRC cells and was more effective than paclitaxel.

Fig. 3

A Flow cytometry using Annexin V-FITC/PI double staining showed UTD1 triggered apoptosis in RKO cells. B Cells were preincubated with or without Z-VAD-FMK (50 µm) for 2 h, and then treated with 1 µg/ml UTD1. Apoptosis was detected by flow cytometry. C Statistical analysis of A and B. D Cleaved caspase-3 was detected after RKO cells were cultured with UTD1 using immunofluorescence staining. Scale bar = 20 μm. E RKO cells were incubated with UTD1 in the presence or absence of 50 μm Z-VAD-FMK. PARP was analyzed by Western blotting. F After exposure to UTD1, TEM was used to evaluate apoptosis. Scale bar = 2 μm. G After 1 µg/ml paclitaxel or UTD1 treated, flow cytometry was used to detect apoptosis of RKO cells. H After exposure to 50 µg/ml UTD1 or paclitaxel 24 h, flow cytometry result showed apoptosis in HCT15 cells. All experiments were performed in triplicate. Results were presented as mean ± SD. ns: no statistically significant difference, **p < 0.01, ***p < 0.001, ****p < 0.0005 vs. control group.