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. 2021 Apr 1;12(4):338. doi: 10.1038/s41419-021-03619-6

Fig. 4. UTD1 was more effective than 5-FU in vitro.

Fig. 4

A RKO cells were cultured with different concentrations of UTD1 and 5-FU for 72 h. Cell viability was measured by CCK-8. B Morphology of RKO cells treated with 1 µg/ml UTD1 and 1 µg/ml, 5 µg/ml 5-FU for 12 h. Scale bar = 50 μm. C Annexin V-FITC/PI staining showed UTD1 and 5-FU induced apoptosis in RKO cells. D CCLE database and qRT-PCR showed expression of ABCB1 in RKO, SW620, CACO2, and HCT15 cells. E SW620, CACO2, and HCT15 cells were treated with different concentrations of UTD1 and 5-FU, and cell viability was measured by CCK-8. F After being treated with 50 µg/ml UTD1 or 5-FU for 36 h, flow cytometry was performed to test apoptosis of HCT15 cells. All experiments were performed in triplicate. Results were presented as mean ± SD, *p < 0.05, **p < 0.01, ****p < 0.0005 vs. control group.