Skip to main content
. 2021 Apr 1;11:7397. doi: 10.1038/s41598-021-86956-y

Figure 2.

Figure 2

The role of CXCL12 treatment in vessel formation of vascular endothelial cells. Representative images showing tube formation of HUVEC (A,B) or HEMEC (C,D) in the absence or presence of CXCL12 (100 ng/ml or 200 ng/ml) for 48 h. Scale Bar; 200 μm. The total number of tube loops was quantified in graphs shown in (B) and (D). Data are from 3 independent experiments, and analyzed using unpaired Student t test analysis including p values (*< 0.05, **< 0.01, ***< 0.001, NS; not significant). (E) A micro-engineered vascular system to detect the angiogenic effect of CXCL12. Representative confocal microscopic images of angiogenic sprouting of HUVECs in response to CXCL12. Scale bar: 100 μm. The total surface area of HUVEC sprouting was quantified in a graph shown in (F). Data shown in (F) are from 3 independent experiments and analyzed using the ordinary one-way ANOVA analysis with Dunnett’s multiple comparison test including P values (*< 0.05, **< 0.01, ***< 0.001, ****< 0.0001, NS; not significant).