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. 2021 Apr 1;11:7360. doi: 10.1038/s41598-021-86522-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Higher level of mtdsRNA in the cytoplasm of CD+ keratocytes compared to in that of CD− keratocytes. (A) The relative level of mtDNA in the pellet fraction (PF) or cytosolic fraction (CF) measured by multiplex qPCR (n = 3). (B) Relative mtRNA in whole-cell lysate (WCL) and CF of CD+/− keratocytes measured by multiplex qPCR (n = 3). (C) Relative mtRNA in CF of CD+ keratocytes 24 h after 10 μM mitoTEMPO treatment (n = 3). (D) Mitochondrial RNA level with/without Terminator 5′-phosphate-dependent exonuclease treatment (TE) in WCL and CF (n = 3). (E) Representative fluorescence image of dsRNA in CD+/− cells. mitoTracker (red), J2 foci (greed) and DAPI (blue) were merged in the right column (merged). Values are means ± SD. n.s. (not significant); *P < 0.05, **P < 0.01.