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. 2021 Apr 1;11:7360. doi: 10.1038/s41598-021-86522-6

Table 4.

Primers and probes used for multiplex qPCR real‐time PCR.

Primer name Sequence (5′–3′)
7SF CTAAATAGCCCACACGTTCCC
7SR AGAGCTCCCGTGAGTGGTTA
ND4F CTGTTCCCCAACCTTTTCCT
ND4R CCATGATTGTGAGGGGTAGG
Fβ2M GCTGGGTAGCTCTAAACAATGTATTCA
Rβ2M CD quantification primers1 CCATGTACTAACAAATGTCTAAAATGGT
HSSN8416 CCTTACACTATTCCTCATCACC
HSAS8542 TGTGGTCTTTGGAGTAGAAACC
Probe name Sequence (5′–3′)
7SP 6FAM-CATCACGATGGATCACAGGT(NFQ)
ND4P Texas Red-GACCCCCTAACAACCCCC(NFQ)
Pβ2MP (reference gene) VIC-CAGCAGCCTATTCTGC(NFQ)
CD Texas Red-TGGCAGCCTAGCATTAGCAGTT(NFQ)72