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. 2021 Apr 1;11:7430. doi: 10.1038/s41598-021-86435-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic reconstruction of Orbiviruses ML, 1 million, 780 nt of the VP4 protein. Reconstruction was performed via the Bayesian MCMC approach using MrBayes with the following settings (burn-in, 10%; frequency, 200; chain length, 1 million to 10 million, depending on when a standard derivation of below 0.025 was reached)⁷⁸. Reconstructed trees were visualized using FigTree and posterior probabilities were depicted for each node (http://tree.bio.ed.ac.uk/software/figtree/).