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. 2021 Apr 1;11:7430. doi: 10.1038/s41598-021-86435-4

Table 2.

Results PCR screening. Bats were screened for the presence of virus nucleic acids by different PCR assays.

PCR assays Assay type Bats
#/novel/size*
Assay reference Virus reference
Adenoviridae
Adenoviridae (Polymerase) Pan 79/14/203H (67) (31)
Arenaviridae
Old-World Pan 0/0/60 (61) Unpublished
Bunyaviridae
Hanta Virus Puumala cPCR 0/0/150 In-house design RKI (71)
Hanta Virus Dobrava cPCR 0/0/150 In-house design RKI (71)
Hanta Virus Tula cPCR 0/0/150 In-house design RKI (71)
Hantaviruses Pan 0/0/180 (64) Unpublished
Nairoviruses Pan 0/0/150 (65) Unpublished
Phleboviruses Pan 0/0/150 (65) Unpublished
Coronaviridae
Coronaviridae Pan 1/1/240 (35) Unpublished (MN851285)
Coronaviridae Pan 0/0/90 In-house design RKI unpublished
Flaviviridae
Flaviviridae Pan 0/0/150 In-house design Unpublished
Flaviviridae Pan 0/0/180 (63) (71)
Herpesviridae
BatGHV1 cPCR 1/1/180 (34) (71)
BatGHV3 cPCR 7/1/180 (34) (71)
BatGHV4 cPCR 22/1/210 (34) (71)
BatGHV5 cPCR 11/1/210 (34) (71)
BatGHV6 cPCR 24/1/210 (34) (71)
BatGHV7 cPCR 2/1/210 (34) (71)
BatBHV1 cPCR 1/1/180 (34) (71)
Herpesviridae pan 11/9/60 (68) Unpublished
Orthomyxoviridae
Influenza A qPCR 0/0/330 (66)
Paramyxoviridae
Res-Mor-Hen Pan 2/2/120 (60) (32)
PAR Pan 3/3/180 (60) (32)
Poxviridae
Low GC poxviruses Pan 0/0/263 (70) Unpublished

pan generic family-specific assay, qPCR quantitative real-time PCR, cPCR conventional specific PCR. *#/novel/size: #, number of positive samples; novel, number of novel viruses obtained; size, bat sample size. HScreened in collaboration with our Hungarian colleagues (see Ref.33).