. 2021 Apr 1;5:7. doi: 10.1038/s41538-021-00086-3

Table 4.

Total (free + esterified) and free oxylipin concentrations in soybean oil heated for 24 h (pmol/μL).

Heating time (h)	0	1	4	8	24
Total 9-HODE	52.77 ± 6.76^a	50.84 ± 4.98^a	65.44 ± 9.88^a	88.53 ± 12.16^b	235.56 ± 71.02^b
Total 13-HODE	61.68 ± 8.65^a	62.44 ± 6.12^a	76.03 ± 12.00^a	98.37 ± 12.33^b	411.24 ± 178.99^ab
Total 9-oxo-ODE	17.24 ± 0.82^a	25.99 ± 3.06^b	37.71 ± 3.35^c	56.52 ± 7.02^d	94.95 ± 39.48^abcd
Total 13-oxo-ODE	28.21 ± 1.56^a	31.64 ± 4.53^a	39.69 ± 4.54^b	54.44 ± 7.98^c	75.44 ± 27.34^abc
Total 9(10)-EpOME	348.97 ± 50.30	315.92 ± 40.90	297.93 ± 50.45	355.66 ± 57.07	380.26 ± 136.88
Total 12(13)-EpOME	537.90 ± 55.91	496.95 ± 69.71	477.81 ± 66.46	556.23 ± 87.97	642.86 ± 199.27
Total 9,10-DiHOME	64.06 ± 2.31	60.53 ± 3.83	64.46 ± 7.37	66.77 ± 7.53	62.37 ± 20.60
Total 12,13-DiHOME	25.74 ± 1.09	24.69 ± 2.01	26.58 ± 3.61	28.06 ± 4.05	33.12 ± 10.00
Total 9,10,13-TriHOME	NQ¹	NQ	NQ	NQ	NQ
Total 9,12,13-TriHOME	NQ	NQ	NQ	NQ	NQ
Σ Total LA-derived oxylipins	1136.57 ± 101.43^a	1069 ± 115.67^a	1085.64 ± 134.95^a	1304.58 ± 154.4^ab	1935.8 ± 299.23^b
Total 9-HOTrE	6.37 ± 0.87^ab	5.78 ± 0.51^a	6.63 ± 1.06^ab	7.79 ± 0.88^b	22.39 ± 8.74^ab
Total 13-HOTrE	4.43 ± 0.75^a	4.93 ± 0.59^a	6.58 ± 0.81^b	9.14 ± 1.16^c	41.54 ± 24.51^abc
Σ Total ALA-derived oxylipins	10.79 ± 1.57^a	10.70 ± 0.99^a	13.21 ± 1.81^a	16.94 ± 1.97^b	63.93 ± 33.18^ab
Free 9-HODE	0.37 ± 0.19	0.39 ± 0.10	0.43 ± 0.18	0.40 ± 0.08	0.50 ± 0.11
Free 13-HODE	0.61 ± 0.24	0.58 ± 0.14	0.80 ± 0.28	0.81 ± 0.17	1.54 ± 0.64
Free 9-oxo-ODE	0.39 ± 0.06	0.38 ± 0.10	0.42 ± 0.03	0.43 ± 0.07	0.57 ± 0.14
Free 13-oxo-ODE	ND²	ND	ND	ND	ND
Free 9(10)-EpOME	0.18 ± 0.07	0.19 ± 0.02	0.21 ± 0.04	0.39 ± 0.35	0.51 ± 0.19
Free 12(13)-EpOME	0.39 ± 0.08^a	0.45 ± 0.03^a	0.52 ± 0.06^a	0.98 ± 0.78^ab	2.30 ± 0.67^b
Free 9,10-DiHOME	0.30 ± 0.28	0.24 ± 0.05	0.27 ± 0.13	0.19 ± 0.04	0.18 ± 0.05
Free 12,13-DiHOME	0.24 ± 0.18	0.20 ± 0.01	0.24 ± 0.08	0.19 ± 0.05	0.16 ± 0.03
Free 9,10,13-TriHOME	0.12 ± 0.05	0.14 ± 0.03	0.14 ± 0.05	0.11 ± 0.02	0.11 ± 0.03
Free 9,12,13-TriHOME	ND	ND	ND	ND	ND
Σ Free LA-derived oxylipins	2.60 ± 1.11^ab	2.57 ± 0.4^ab	3.04 ± 0.73^ab	3.50 ± 1.03^a	5.86 ± 1.85^b
Free 9-HOTrE	ND	ND	ND	ND	ND
Free 13-HOTrE	ND	ND	ND	ND	ND
Σ Free ALA-derived oxylipins	NA²	NA	NA	NA	NA
%Free oxylipins	0.23 ± 0.11	0.24 ± 0.05	0.28 ± 0.08	0.27 ± 0.10	0.30 ± 0.11

Data are expressed at mean ± SD of n = 5 per time-point.

¹NQ: not quantified because the d4-PGE2 surrogate standard used to quantify the analyte degraded during hydrolysis.

²ND: not detected, or NA: non-applicable.

^a–dDifferent letters within a row are significantly different by repeated-measures one-way ANOVA followed by Tukey’s post-hoc test (p < 0.05). LA linoleic acid, ALA α-linolenic acid.