Table 5.

Velocity (v, pmol·μL⁻¹·min⁻¹) and turnover (F, ×10^–8·min⁻¹) of total, free and esterified oxylipins in soybean oil heated for 24 h.

Oxylipins	v (Total)	v (Esterified)	v (Free)	F (Total)	F (Esterified)	F (Free)
9-HODE	0.13 ± 0.054	0.13 ± 0.05	0.0001 ± 0.0001*	9.12 ± 3.48	9.17 ± 3.49	0.85 ± 1.24*
13-HODE	0.25 ± 0.13	0.25 ± 0.13	0.0006 ± 0.0005*	17.53 ± 9.22	17.60 ± 9.25	6.37 ± 4.69
9-oxo-ODE	0.052 ± 0.028	0.052 ± 0.028	0.0001 ± 0.0001*	3.63 ± 1.93	3.64 ± 1.94	1.33 ± 1.14
13-oxo-ODE	0.032 ± 0.019	0.032 ± 0.019	NQ 1	2.25 ± 1.29	2.27 ± 1.30	NQ
9(10)-EpOME	0.040 ± 0.082	0.039 ± 0.082	0.0002 ± 0.0002	2.69 ± 5.45	2.70 ± 5.49	2.18 ± 1.56
12(13)-EpOME	0.097 ± 0.14	0.095 ± 0.14	0.0014 ± 0.0005	6.61 ± 9.53	6.56 ± 9.62	13.74 ± 4.74
9,10-DiHOME	−0.0002 ± 0.014	−0.0002 ± 0.014	−0.0001 ± 0.0001	−0.0002 ± 0.0094	−0.0001 ± 0.0095	−0.0065 ± 0.0091
12,13-DiHOME	0.0056 ± 0.0069	0.0056 ± 0.0069	−0.0001 ± 0.0001	0.39 ± 0.49	0.40 ± 0.49	−0.0058 ± 0.0082
9,10,13-TriHOME	NQ	NQ	−0.00002 ± 0.00002	NQ	NQ	−0.0017 ± 0.0024
9,12,13-TriHOME	NQ	NQ	NQ	NQ	NQ	NQ
ΣLA-derived oxylipins	0.61 ± 0.20	0.61 ± 0.20	0.0023 ± 0.0015*	42.20 ± 12.38	42.33 ± 12.47	23.31 ± 14.74
9-HOTrE	0.012 ± 0.0067	0.012 ± 0.0067	NQ	6.65 ± 3.62	6.70 ± 3.63	NQ
13-HOTrE	0.027 ± 0.018	0.027 ± 0.018	NQ	15.01 ± 9.85	15.12 ± 9.90	NQ
ΣALA-derived oxylipins	0.038 ± 0.025	0.038 ± 0.025	NQ	21.65 ± 13.45	21.81 ± 13.51	NQ

Velocity and turnover data are expressed at mean ± SD of n = 5 per oxylipin within each lipid fraction (total, esterified and free).

¹NQ: not quantified because the PGE2 surrogate standard used to quantify the analyte degraded during hydrolysis (for esterified TriHOMEs), or a slope was not determined from the Concentration-Time plot due to non-detected values over time (free 13-oxo-ODE, 9,12,13-TriHOME, 9-HOTrE, and 13-HOTrE).

*Velocity or turnover of esterified oxylipin is significantly different from that of free oxylipin (p < 0.05) by unpaired t-test.