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. 2021 Apr 1;11:7347. doi: 10.1038/s41598-021-86762-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of apoptosis induction. Annexin V-FITC analysis of (a) BT-474, (b) SK-BR-3, and (c) MDA-MB-231 cells untreated and treated with PTXNR-TTZ for 0, 24, 48, and 72 h. Results represent one of three independent experiments. Viable cells are represented in the lower left quadrant. Early apoptosis (Annexin + / PI −) is shown in the lower right quadrant for each panel, while late apoptosis (Annexin + / PI +) is shown in the upper right quadrant. Necrosis (Annexin − / PI +) is shown in the upper left quadrant. Values are presented as means ± SD of triplicate experiments. Detailed statistical analysis (p < 0.05) of the data are shown in SI Table 4.