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. 2021 Apr 1;12:2034. doi: 10.1038/s41467-021-22110-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of the outer coat lozenge architecture, with left-handed rod positions highlighted in the box. b Top panel: top view of the 11 Å subtomogram average of the COPII left-handed rod, with rigid-body fitted models (PDB 2PM6). The dimerising protomers are shown in dark and light pink, Sec13 in grey. Bottom panel: side view of (b). c Details of the map in (b), showing the quality of the fit. Left panel: detail of Sec13 β-propeller. Right panel: detail of Sec31 α-solenoid domain. Sigma threshold for contouring was set at 5 for (b, c). d Enlargement of the region boxed in panel ‘b’ shown at lower contour (sigma = 2.1) to display an extra density.