Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 1;12:2034. doi: 10.1038/s41467-021-22110-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a The interface between neighbouring inner coat subunits viewed from the top. A dotted line defines the boundary between two protomers. b Enlarged view of the Sec23-Sar1 interface, with the modelled Sec23 17-residue L-loop in dark cyan. This loop is disordered in the X-ray structure, but becomes ordered in the lattice, mediating interactions with Sar1. Sigma threshold for contouring was set at 0.08 for (a, b). c GUV budding reconstitutions in the presence of wild-type Sec31 and Sec23 lacking the L-loop show straight tubules, similar to wild-type reactions. d Budding creates spherical profiles when Sec31 has all of its PPP sites mutated to alanine to further weaken inner coat assembly. e Floppy tubules form when, in addition to the conditions in ‘d’, Sec31 vertex interactions are destabilised with Nhis-Sec31. Scale bar 100 nm. Micrographs in (c–e) are representative of experiments repeated at least three times.