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. 2021 Apr 1;12(4):347. doi: 10.1038/s41419-021-03641-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Volcano plot of the gene expression changes that occurred after LDHA knockdown in B-CPAP cells assayed by RNA-Seq. B Summary of the significantly enriched pathways in B-CPAP cells after LDHA knockdown. C Heatmap from the RNA-Seq data showing the differentially expressed genes involved in the EMT process. D qPCR analysis of representative genes related to the EMT process after LDHA knockdown. The expression data were normalized to that of Actin in GFP knockdown cells. E Heatmap of the RNA-Seq data show that the differentially expressed genes were related to the mitochondrial respiratory chain complex. F qPCR analysis of representative genes related to the mitochondrial respiratory chain complex after LDHA knockdown. All *p < 0.05, **p < 0.01, ***p < 0.001.