Table 1.

Analytical conditions employed for acrylamide content analysis

Items	Conditions
Chromatograph	Shimadzu 30A
Column	Kinetex polar C18 (150 mm × 2.1 mm i.d., 2.6 μm particle size, Phenomenex)
Flow rate	0.3 mL/min
Oven temperature	26 °C
Injection volume	20 μL
Mobile phases	0.5% methanol in distilled water and 0.1% acetic acid
Mass spectrometer	Shimadzu MS8040
Ionization mode	Electrospray ionization, 5,000 V, positive mode
Detection mode	Multiple reaction monitoring (MRM)
Desolvation gas, collision gas	N2
MRM ions	Acrylamide (m/z 72 → 55, 72 → 27), Internal standard (m/z 75 → 58, 72 → 29)