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. 2021 Mar 19;12:543767. doi: 10.3389/fmicb.2021.543767

TABLE 2.

The Adonis analysis using unweighted Unifrac distance among the groups.

Categories F R2 p
(1) All samples
Type (Stomach vs. Gut) 11.523 0.204 0.001
Sampling time (Day 80 vs. Day 330) 13.398 0.229 0.001
Temperature (5, 15, and 25 degrees) 5.071 0.101 0.001
Sampling time * Temperature 6.909 0.457 0.001
Sampling time * Type 13.603 0.486 0.001
Temperature * Type 5.577 0.405 0.001
Sampling time * Temperature * Type 10.784 0.772 0.001
(2) Stomach
Sampling time (Day 80 vs. Day 330) 12.498 0.385 0.001
Temperature (5, 15, and 25 degrees) 3.765 0.158 0.010
(3) Gut
Sampling time (Day 80 vs. Day 330) 11.270 0.329 0.001
Temperature (5, 15, and 25 degrees) 5.574 0.195 0.001

Adonis: Partitions a distance matrix among sources of variation in order to describe the strength and significance that a categorical or continuous variable has in determining variation of distances (http://qiime.org/scripts/compare_categories.html). Here, the variables include sampling time, type, and temperature. (1) All samples: All 47 samples were used in the analysis. (2) Stomach: Only the samples from the stomach were used in analysis. (3) Gut: Only the samples from the gut were used in analysis.