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. 2021 Mar 19;12:635843. doi: 10.3389/fmicb.2021.635843

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Copyright © 2021 Maciel, Gonçalves, Antonelli and Fonseca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine profile of mice fed with different experimental diets. Mice received different restricted diets: Control (Ctrl), Low protein 3 and 8% (LP 3% and LP 8%, respectively). Serum samples were obtained before infection (∼day 50), and before perfusion (∼day 100) for cytokine measurement. Levels of IFN-γ (A), TNF (B), IL-6 (C), IL-2 (D), IL-4 (E), IL-10 (F), and IL-17 (G) production were measured using the CBA Th1/Th2/Th17 kit. BI: before infection; BP: before perfusion. Results are representative of two independent experiments (n = 6–11 mice per group) and significant differences were determined by one-way ANOVA followed by Holm-Sidak’s multiple comparison test (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001).