TABLE 1.
The catalytic activities and kinetic parameters of the five typical catalases from S. rimosus ATCC 10970.
| Name | Protein ID | Molecular weight (kDa) | Specific activity * (×105 U/mg holoenzyme) | Km (mM) | kcat * (×105 s–1) | kcat/Km * (×104 s–1 mM–1) |
| Cat1 | QEV74945.1 | 56.69 | 1.53 ± 0.08 | 53.5 ± 9.4 | 6.55 ± 0.72 | 1.22 ± 0.25 |
| Cat2 | QEV75671.1 | 64.95 | 2.21 ± 0.03 | 24.8 ± 4.3 | 6.09 ± 0.51 | 2.46 ± 0.48 |
| Cat3 | QEV75878.1 | 56.63 | 1.70 ± 0.20 | 35.1 ± 4.5 | 5.13 ± 0.36 | 1.46 ± 0.22 |
| Cat4 | QEV76009.1 | 57.46 | 2.24 ± 0.10 | 20.1 ± 1.4 | 4.70 ± 0.15 | 2.33 ± 0.18 |
| Cat5 | QEV79658.1 | 79.43 | 0.237 ± 0.006 | 44.7 ± 6.3 | 1.24 ± 0.11 | 0.276 ± 0.046 |
*The specific activities and kinetic parameters were calculated based on the concentrations of holoenzymes (deduced from the detected heme occupancy of each purified catalase).