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. 2021 Mar 19;9:653724. doi: 10.3389/fcell.2021.653724

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Copyright © 2021 Xu, Yang, Ye, Chen, Zhang, Wu, Song, Feng, Feng, Chen, Wang, Lin, Bai and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of si-SPTBN1 and OE-SPTBN1 in the Smad3/TGF-β and STAT1/Cxcl-9 pathways in vitro. (A) MC3T3-E1 cells were transfected with si-SPTBN1 or OE-SPTBN1 for 1, 3, 5, 7, and 14 days, then the protein expression of SPTBN1, Runx2, TGF-β and p-Smad3 was evaluated by Western blot. (B) MC3T3-E1 cells were transfected with si-SPTBN1 or OE-SPTBN1 for 1, 3, 5, 7, and 14 days, then the protein expression of SPTBN1 and p-STAT1 was evaluated by Western blot. β-actin was considered as the internal reference. (C) The expression of Cxcl-9 was evaluated by ELISA assay. N = 3, ^∗P < 0.05, ^∗∗P < 0.01 vs. control group.