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. 2021 Mar 19;11:643913. doi: 10.3389/fcimb.2021.643913

Figure 4.

Figure 4

The effect of P. aeruginosa and F. nucleatum on IL-1β, IL-6 and TNF-α productions. (A) The effect of P. aeruginosa on IL-1β, IL-6 and TNF-α productions of pulmonary epithelial cells. A549 cells were infected with different MOI (10, 50, 100) of P. aeruginosa for 24 h. (B) The effect of F. nucleatum on IL-1β, IL-6 and TNF-α productions of pulmonary epithelial cells. A549 cells were infected with different MOI (10, 50, 100) of F. nucleatum for 24 h. (C) The effect of the simultaneous infection of P. aeruginosa and F. nucleatum on IL-1β, IL-6 and TNF-α productions of pulmonary epithelial cells. A549 cells were simultaneously infected with P. aeruginosa (MOI 100) and F. nucleatum (MOI 100) for 24 h, and the single P. aeruginosa or F. nucleatum infection groups (MOI 100, 200) were used as control. *P < 0.05, statistics were achieved by analysis of variance followed by Sidak’s multiple comparisons test. Pa, P. aeruginosa; Fn, F. nucleatum.