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. 2021 Mar 19;11:643913. doi: 10.3389/fcimb.2021.643913

Figure 6.

Figure 6

The effect of F. nucleatum pretreatment on cellular proliferation, cytotoxicity and inflammation. (A) Schematic representation of cell assay. A549 cells cultured in DMEM for 24 h were the blank control. Pa represented A549 cells precultured in DMEM for 12 h followed by P. aeruginosa (MOI 100) infection for other 12 h. Fn represented A549 cells treated with F. nucleatum (MOI 100) for 24 h. Fn-Fn means that A549 cells pretreated with F. nucleatum (MOI 100) for 12 h continue to be infected with another F. nucleatum (MOI 100) for 12 h. Fn-Pa means that A549 cells pretreated with F. nucleatum (MOI 100) for 12 h continue to be infected with P. aeruginosa (MOI 100) for 12 h. (B) The effect of F. nucleatum pretreatment on cellular proliferation of pulmonary epithelial cells. (C) The effect of F. nucleatum pretreatment on cytotoxicity of pulmonary epithelial cells. (D) The effect of F. nucleatum pretreatment on IL-1β, IL-6 and TNF-α productions of pulmonary epithelial cells. *P < 0.05, statistics were achieved by analysis of variance followed by Sidak’s multiple comparisons test.