FIGURE 1.

N-terminomic analysis of ADAMTS2 and 14 in mouse skin. (A) Schematic view of the experimental method to assess the potential substrates of ADAMTS2 and/or ADAMTS14 in mouse skin and to investigate their biological function in vivo. Three independent iTRAQ-TAILS analyses using skin proteome of each genotypes (4-plex) have been performed. As a general procedure, a protease/control (P/C) ratio above or below the three sigma cutoff from the normal distribution of natural N-termini is considered to be related to the studied protease. Here, the ratio Wt/TS2^−/− and TS14^−/−/TS2^−/−TS14^−/− were used to investigate the ADAMTS2 substrates, the ratios Wt/TS14^−/− and TS2^−/−/TS2^−/−TS14^−/− were used to investigate the ADAMTS14 substrates and the ratio Wt/TS2^−/−TS14^−/− was used to investigate the substrates common to ADAMTS2 and ADAMTS14. (B) Venn diagram showing the number of specific or common total (left) or labeled (right) proteins identified in each experiment. Experiment A is in blue, B in green and C in purple. Venn diagrams were generated using the online BioVenn software (Hulsen et al., 2008).