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. 2021 Mar 19;8:643178. doi: 10.3389/fmolb.2021.643178

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Copyright © 2021 Leduc, Dupont, Joannes, Monseur, Baiwir, Mazzucchelli, Deroanne, Colige and Bekhouche.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Collagen digestion from calf skin by recombinant human ADAMTS2 and/or ADAMTS14 (A–F) Collagen from dermatospatactic calf (DC) skin has been heat denatured (10 min at 95°C) (B, D, F) or not (A, C, E) before addition of recombinant human TS2 and/or TS14 overnight at 37°C. Digestion products have been analyzed by 6.25% (A, B) or 10% (C–F) SDS-PAGE stained by Coomassie blue. Pictures at (E) and (F) correspond respectively to (C) and (D) with a higher contrast to emphasize the degradation trail. Collagen from normal calf skin (NC) have been used as control for the identification of the fully processed α1 and α2 chains. Products at 31 kDa (C, E) correspond to the N-terminal propeptide released mainly by ADAMTS2 when type I collagen is in its native form. Additional low MW products can be observed after incubation of denatured collagen with ADAMTS14 and ADAMTS2.