FIGURE 5.

Cleavage of type I procollagen. The collagen substrate, mainly in the form of pro-α1I and pro-α2I, was recovered from culture medium conditioned by dermatosparactic fibroblasts, and then incubated with recombinant human ADAMTS2 and/or ADAMTS14 during 18 h at 37°C. Digestion products were analyzed by 6.25% SDS-PAGE stained by Coomassie blue. The cleavage of pro-α2 into pCα2 and mature α2 demonstrates that ADAMTS2 displays a procollagen C-proteinase activity in addition to its aminoprocollagen peptidase property.