Skip to main content
. 2021 Mar 19;8:643178. doi: 10.3389/fmolb.2021.643178

FIGURE 6.

FIGURE 6

In vitro cleavage of actin and vimentin by ADAMTS2 and ADAMTS14. Table showing the P/C ratios according to the cleavage sites observed by proteomic. Ratios above the average 3σ cut-offs are highlighted in green (A). Western blot analysis of in vitro cleavage of actin (B) and vimentin (C) by ADAMTS2 and/or ADAMTS14. The schematic representation of actin and vimentin are reported on the top of the western blots with their respective actinin binding (magenta) and coiled coil (blue) domains. Human fibroblasts lysates were incubated in the presence of recombinant ADAMTS2 and/or ADAMTS14 (200 nM, 16 h at 37°C). EDTA was used as an inhibitor of metalloproteinases. Degradation products are indicated by black arrows.