TABLE 2.
Type I collagen processing in mouse skin.
| Collagen α1(I) chain | Collagen α2(I) chain | ||||||
|---|---|---|---|---|---|---|---|
| Peptide Sequence | TS2 | TS14 | TS2TS14 | Peptide Sequence | TS2 | TS14 | TS2TS14 |
| NC2 (A150-P167) | NC2 (A75-P96) | ||||||
| NFAS(151).(152)QMSYGYDEKSAGVSVPGPMGPSGPR | 4.2 ± 0.5 | 1.7 ± 0.2 | 6.8 ± 1.0 | NFAA(85).(86)QYSDKGVSSGPGPMGLMGPR | 3.1 ± 0.7 | 1.6 ± 0.2 | 4.6 ± 0.6 |
| FASQ(152).(153)MSYGYDEKSAGVSVPGPMGPSGPR | 2.0 ± 0.3 | 1.5 ± 0.2 | 2.9 ± 0.4 | FAAQ(86).(87)YSDKGVSSGPGPMGLMGPR | 2.0 ± 0.3 | 2.0 ± 0.3 | 1.5 ± 0.2 |
| QMSY(155).(156)GYDEKSAGVSVPGPMGPSGPR | 2.4 ± 1.0 | 1.6 ± 0.3 | 3.3 ± 1.1 | YSDK(90).(91)GVSSGPGPMGLMGPR | 2.9 ± 0.4 | 1.4 ± 0.2 | 2.9 ± 0.6 |
| SYGY(157).(158)DEKSAGVSVPGPMGPSGPR | 3.0 ± 1.0 | 1.8 ± 0.3 | 5.2 ± 2.0 | SDKG(91).(92)VSSGPGPMGLMGPR | 1.9 ± 0.3 | 1.4 ± 0.2 | 2.6 ± 0.3 |
| DEKS(161).(162)AGVSVPGPMGPSGPR | 2.0 ± 0.3 | 1.5 ± 0.2 | 2.9 ± 0.5 | DKGV(92).(93)SSGPGPMGLMGPR | 1.9 ± 0.2 | 1.5 ± 0.1 | 2.8 ± 0.1 |
| EKSA (162).(163)GVSVPGPMGPSGPR | 1.9 ± 0.3 | 1.4 ± 0.1 | 2.6 ± 0.4 | Triple helix (G97-G1113) | |||
| KSAG(163).(164)VSVPGPMGPSGPR | 3.7 ± 0.3 | 1.4 ± 0.1 | 5.1 ± 0.2 | SSGP(96).(97)GPMGLMGPR | 1.9 ± 0.3 | 1.5 ± 0.2 | 2.7 ± 0.5 |
| Triple helix (<I>G168-P1181</I>) | GPGP(98).(99)MGLMGPR | 2.5 ± 0.8 | 1.8 ± 0.5 | 3.9 ± 1.6 | |||
| VSVP(167).(168)GPMGPSGPR | 1.8 ± 0.5 | 1.6 ± 0.4 | 2.5 ± 0.6 | RGIP(336).(337)GPAGAAGATGAR | 2.5 ± 0.7 | 1.7 ± 0.4 | 3.6 ± 1.0 |
| VPGP(169).(170)MGPSGPR | 1.8 ± 0.5 | 1.5 ± 0.4 | 2.6 ± 1.0 | RPGP(485).(486)IGPAGPR | 2.4 ± 0.7 | 1.6 ± 0.4 | 3.5 ± 1.3 |
| TGPP(332).(333)GFPGAVGAKGEAGPQGAR | 2.1 ± 0.6 | 1.7 ± 0.3 | 3.5 ± 1.1 | RGTP(600).(601)GESGAAGPSGPIGSR | 2.1 ± 0.6 | 1.6 ± 0.3 | 3.2 ± 1.1 |
| PPGF(334).(335)PGAVGAKGEAGPQGAR | 2.3 ± 0.8 | 1.7 ± 0.5 | 3.5 ± 1.7 | GESG(604).(605)AAGPSGPIGSR | 2.0 ± 0.2 | 1.3 ± 0.1 | 2.5 ± 0.4 |
| PGFP(335).(336)GAVGAKGEAGPQGAR | 2.4 ± 0.8 | 1.9 ± 0.5 | 4.0 ± 1.3 | ESGA(605).(606)AGPSGPIGSR | 2.0 ± 0.4 | 1.4 ± 0.2 | 2.7 ± 0.5 |
| RGFP(485).(486)GADGVAGPKGPSGER | 2.6 ± 1.0 | 1.7 ± 0.4 | 3.8 ± 1.5 | VGAP (636).(637)GSAGASGPGGLPGER | 2.3 ± 0.8 | 1.7 ± 0.4 | 3.5 ± 1.2 |
| GFPG(486).(487)ADGVAGPKGPSGER | 1.8 ± 0.3 | 1.4 ± 0.2 | 2.3 ± 0.2 | SGDR(699).(700)GEAGAAGPSGPAGPR | 1.9 ± 0.4 | 1.6 ± 0.3 | 3.0 ± 0.7 |
| AGAQ(608).(609)GAPGPAGPAGER | 2.2 ± 0.4 | 1.6 ± 0.3 | 3.1 ± 0.3 | GDRG(700).(701)EAGAAGPSGPAGPR | 1.9 ± 0.3 | 1.5 ± 0.2 | 2.8 ± 0.3 |
| PGPI(842).(843)GNVGAPGPKGPR | 2.4 ± 0.3 | 1.9 ± 0.3 | 4.3 ± 0.2 | DRGE(701).(702)AGAAGPSGPAGPR | 2.4 ± 0.6 | 1.7 ± 0.4 | 3.8 ± 1.1 |
| PPGP(889).(890)VGKEGGKGPR | 2.4 ± 0.6 | 1.4 ± 0.3 | 3.1 ± 0.8 | GEAG(703).(704)AAGPSGPAGPR | 2.0 ± 0.2 | 1.3 ± 0.1 | 2.5 ± 0.4 |
| AGSP(935).(936)GTPGPQGIAGQR | 2.2 ± 0.6 | 1.6 ± 0.3 | 3.1 ± 1.0 | EAGA(704).(705)AGPSGPAGPR | 2.1 ± 0.4 | 1.5 ± 0.2 | 3.0 ± 0.7 |
| PGTP(938).(939)GPQGIAGQR | 2.6 ± 0.8 | 1.6 ± 0.4 | 3.8 ± 1.3 | AGAP(969).(970)GPHGSVGPAGKHGNR | 2.2 ± 0.5 | 1.6 ± 0.3 | 3.4 ± 0.7 |
| KNGD(1,054).(1,055)RGETGPAGPAGPIGPAGAR | 2.1 ± 0.5 | 2.0 ± 0.4 | 3.6 ± 0.1 | RGEP(987).(988)GPAGSVGPVGAVGPR | 2.5 ± 0.8 | 1.6 ± 0.4 | 3.7 ± 1.4 |
| NGDR(1,055).(1,056)GETGPAGPAGPIGPAGAR | 2.0 ± 0.4 | 1.4 ± 0.2 | 2.6 ± 0.5 | EPGP(989).(990)AGSVGPVGAVGPR | 2.2 ± 0.6 | 1.5 ± 0.3 | 3.2 ± 1.1 |
| RGET (1,058).(1,059)GPAGPAGPIGPAGAR | 1.7 ± 0.2 | 1.2 ± 0.1 | 2.1 ± 0.1 | PGPA(990).(991)GSVGPVGAVGPR | 1.9 ± 0.2 | 1.3 ± 0.1 | 2.4 ± 0.4 |
| ETGP(1,060).(1,061)AGPAGPIGPAGAR | 2.4 ± 0.7 | 1.6 ± 0.4 | 3.5 ± 1.1 | AGSV(993).(994)GPVGAVGPR | 2.3 ± 0.5 | 1.5 ± 0.2 | 3.3 ± 0.9 |
| PAGP(1,063).(1,064)AGPIGPAGAR | 2.6 ± 0.8 | 1.5 ± 0.4 | 3.7 ± 1.5 | SVGP(995).(996)VGAVGPR | 1.7 ± 0.2 | 1.6 ± 0.2 | 2.6 ± 0.3 |
| NC1 (S1182-V1453) | LKGY(1,031).(1,032)SGLQGLPGLAGLHGDQGAPGPVGPAGPR | 2.2 ± 0.6 | 1.9 ± 0.5 | 3.6 ± 1.1 | |||
| GYDF(1,187).(1,188)SFLPQPPQEKSQDGGR | 4.4 ± 0.4 | 9.5 ± 3.6 | 24.3 ± 7.6 | YSGL(1,034).(1,035)QGLPGLAGLHGDQGAPGPVGPAGPR | 2.6 ± 1.2 | 1.7 ± 0.5 | 3.1 ± 1.0 |
| DTTL(1,224).(1,225)KSLSQQIENIR | 2.5 ± 0.9 | 2.0 ± 0.9 | 3.4 ± 0.8 | GLPG (1,039).(1,040)LAGLHGDQGAPGPVGPAGPR | 2.9 ± 0.6 | 1.9 ± 0.5 | 5.6 ± 2.4 |
| LKSL(1,227).(1,228)SQQIENIR | 2.3 ± 0.6 | 1.7 ± 0.5 | 3.2 ± 0.8 | LPGL(1,040).(1,041)AGLHGDQGAPGPVGPAGPR | 2.3 ± 0.4 | 1.3 ± 0.2 | 2.8 ± 0.3 |
| PGLA(1,041).(1,042)GLHGDQGAPGPVGPAGPR | 2.8 ± 1.2 | 2.5 ± 1.2 | 6.1 ± 2.9 | ||||
| LAGL(1,043).(1,044)HGDQGAPGPVGPAGPR | 2.0 ± 0.3 | 1.6 ± 0.2 | 2.9 ± 0.5 | ||||
| GLHG(1,045).(1,046)DQGAPGPVGPAGPR | 1.5 ± 0.4 | 1.8 ± 0.2 | 2.7 ± 0.9 | ||||
| LHGD(1,046).(1,047)QGAPGPVGPAGPR | 2.2 ± 0.3 | 1.5 ± 0.2 | 3.2 ± 0.1 | ||||
| HGDQ(1,047).(1,048)GAPGPVGPAGPR | 1.8 ± 0.2 | 1.3 ± 0.1 | 2.4 ± 0.4 | ||||
| GDQG(1,048).(1,049)APGPVGPAGPR | 2.0 ± 0.2 | 1.4 ± 0.2 | 2.7 ± 0.5 | ||||
| RSGQ(1,076).(1,077)PGPVGPAGVR | 2.2 ± 0.3 | 1.5 ± 0.2 | 3.2 ± 0.1 | ||||
| NC1 (Y1114-K1372) | |||||||
| DATL(1,145).(1,146)KSLNNQIETLLTPEGSR | 3.9 ± 3.4 | 6.0 ± 5.6 | 3.2 ± 1.0 | ||||
| LKSL(1,148).(1,149)NNQIETLLTPEGSR | 1.6 ± 0.4 | 2.0 ± 0.6 | 2.7 ± 0.8 | ||||
| RLPF(1,347).(1,348)LDIAPLDIGGADQEFR | 3.0 ± 0.7 | 2.0 ± 0.6 | 5.3 ± 1.2 | ||||
Cleavage sites observed by proteomics of ADAMTS2 (TS2) and ADAMTS14 (TS14) within α1 and α2 chains of type I collagen. Each cleavage site has been observed at least in two experiments. The sequences of the cleavage sites are reported in bracket according to the UniprotKB numbering. The classical N-propeptide cleavage sites are highlighted in grey.