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. 2021 Mar 19;11:618994. doi: 10.3389/fcimb.2021.618994

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Copyright © 2021 Ma, Tirtorahardjo, Jan, Schweizer, Rosario, Du, Zhang, Morrissette and Andrade

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Auranofin-resistant T. gondii parasites accumulate less ROS. Freshly lysed wild-type parasites were treated with 500 μM hydrogen peroxide (H₂O₂) for 30 min and accumulation of reactive oxygen species (ROS) was assayed with dichloro-fluorescein H₂DCDFC. Auranofin-resistant clones accumulated less ROS after H₂O₂ treatment compared to the wild-type RH parasites in the presence of H₂O₂ (white) or H₂O₂ + 1 µM auranofin (black). (*) p < 0.05 when compared to RH or to RH+ Auranofin (Au).