Figure 5.

A L201P mutation in TgSOD2 does not alter localization of TgSOD2 or confer resistance to auranofin. (A) Wild type and mutant SOD2-YFP exhibit a mitochondrial distribution. Mitochondrion was stained red with the anti-F1B ATPase. Scale bar: 5 µm. (B) Growth competition assays indicate that the L201P mutation is associated with reduced fitness in the absence of auranofin (control conditions, white bars). In the presence of auranofin (black bars), only line 8 shows growth advantage, indicating that the single L201P mutation to TgSOD2 does not confer resistance. (C) In the absence of auranofin, the SOD2.L201P line accumulated ROS similarly to wild type parasites, consistent with its auranofin-sensitive growth; however, it accumulated less ROS in the presence of auranofin. *p < 0.05 when compared to RH in auranofin (+Au).