Table 1.

SNVs in Aur^R line 8.

Rank	Mutation	Locus	Predicted protein	CRISPR	*RNA-seq	Motif	Index
1	L201P	TGGT1_316330	Superoxide dismutase SOD2	−4.09	76.99	SOD motif	2.50
2	L166Q	TGGT1_294640	Ribonucleoside-diphosphate reductase	−4.79	40.9	R1 subunit, ribonucleotide reductase	2.29
3	Y95C	TGGT1_233460	SAG-related sequence SRS29B	−0.07	1809.46	Major surface antigen p30	2.10
4	Y637H	TGGT1_268870	Tetracopeptide repeat-containing	−1.66	38.52	Predicted dehydrogenase	1.81
5	P1357-S1358Δ	TGGT1_310350	PGAP1 family protein	−2.41	11.67	Alpha/beta-hydrolase/PGAP1-like protein	1.45
6	D487G	TGGT1_289820	TBC domain-containing protein	−1.14	15.49	Rab-GTPase-TBC domain	1.25
7	L606L	TGGT1_271290	Hypothetical protein	−2.02	8.1	Alternative splicing regulator	1.21
8	F424L	TGGT1_234250	Hypothetical protein	−2.02	6.39	SWAP/MAEBL	1.11
9	A574G	TGGT1_290910	Hypothetical protein	−1.39	7.63	No putative conserved domain	1.03
10	D1983del	TGGT1_211310	Hypothetical protein	−0.69	12.86	RCC1 repeat	0.95
11	F563S	TGGT1_260490	Hypothetical protein	−1.62	5.34	Herpes BLLF1	0.94
12	P66P	TGGT1_225340	Hypothetical protein	0.73	24.06	No putative conserved domain	N/A

*Transcriptome Gregory series at 2 h RH (log 2 FPKM+1). FPKM, fragments per kilobase of exon per million fragments mapped; N/A: not calculated due to dispensable (positive) CRISPR phenotype index.

index Formula = Sum of Log10 of (−CRISPR phenotype index)+log10 (RNAseq expression).