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. 2021 Mar 19;12:654623. doi: 10.3389/fimmu.2021.654623

Figure 1.

Figure 1

The phenotype of IL-21-producing CD8+ T cells. (A) PBMCs from HCs were stimulated in the absence or presence of CD3/28 beads for 72 h and then IL-21 production from CD4+ and CD8+ T cells was analyzed by intracellular staining (added PMA and Ionomycin for the last 6 h). Left panels are representative data on IL-21 production from CD4+ and CD8+ T cells stimulated with CD3/28 and the right graph summarizes the results (N=4). (B) PBMCs from HCs were stimulated under the same condition as (A), and IFN-γ and IL-17 production from IL-21+ and IL-21- CD8+ T cells was analyzed by intracellular staining. (C) PBMCs from HCs were stimulated under the same condition as (A), and expression levels of PD-1, ICOS, CD95, CD69, CD28 and HLA-DR in IL-21+ and IL-21- CD8+ T cells were analyzed by flow cytometry. The graph summarizes the results (N=4). *P < 0.05, **P < 0.01, NS, no stimulation.