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. 2021 Mar 19;11:603128. doi: 10.3389/fonc.2021.603128

Figure 3.

Figure 3

HOTAIRM1 upregulation promoted proliferation, invasion, and migration of malignant transformed fibroblasts. (A) HOTAIRM1 expression was analyzed with qRT-PCR in t-FB1/2 transfected with HOTAIRM1 or NC. **p < 0.01, Student’s t test. (B, C) Proliferation ability was evaluated after HOTAIRM1 upregulation in t-FB1/2 using EdU assay. *p < 0.05, Student’s t test. (D, E) Invasion capacity was assessed after HOTAIRM1 upregulation in t-FB1/2 by transwell assay. *p < 0.05, **p < 0.01, Student’s t test. (F, G) Migration ability was evaluated in t-FB1/2 with HOTAIRM1 overexpression by wound healing assay. **p < 0.01, Student’s t test. (H, K) Tumorigenicity was compared in murine subcutaneous tumor model between t-FB1/2 with NC or HOTAIRM1 transfection. (I, J, L, M) Tumor growth curve and tumor weight of NC and HOTAIRM1 transfection group. ****p < 0.0001, two-way ANOVA; **p < 0.01, one-way ANOVA.