Figure 1.
Overview of renal tubule cell nomenclature and experimental design. (A) The scheme12,13 shows the connection of both a short-looped and a long-looped nephron to the CD system. Small-sample RNA-seq coupled with microdissection was used to quantify gene expression in all 14 mouse renal tubule segments. The data are provided in a user-friendly website. (B) Representative images of microdissected mouse renal tubule segments. The images were captured by the Invitrogen EVOS XL Core Cell Imaging System. Scale bars, 200 µm. G, glomerulus; PTS1, the initial segment of the proximal tubule; PTS2, proximal straight tubule in cortical medullary rays; PTS3, last segment of the proximal straight tubule in the outer stripe of outer medulla; DTL1, the short descending limb of the loop of Henle; DTL2, long descending limb of the loop of Henle in the outer medulla; DTL3, long descending limb of the loop of Henle in the inner medulla; ATL, thin ascending limb of the loop of Henle; MTAL, medullary thick ascending limb of the loop of Henle; CTAL, cortical thick ascending limb of the loop of Henle; MD, mecula densa; DCT, distal convoluted tubule; CNT, connecting tubule; CCD, cortical collecting duct; OMCD, outer medullary collecting duct; IMCD, inner medullary collecting duct.