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. 2021 Mar 3;32(4):994–1004. doi: 10.1681/ASN.2020060850

Figure 1.

Figure 1.

Simple collection protocol enables ease of use. Urine samples are collected in a standard urine cup at any time during the day. The exosomes are isolated from the urine sample, followed by an RNA extraction, and RT-qPCR analysis of the target genes. The relative quantities of each target gene are inputted into an algorithm to generate a single score from zero to one.