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. 2020 Dec;63(6):831–842. doi: 10.1165/rcmb.2019-0396OC

Figure 5.

Figure 5.

FOXL1 is related to BMP signaling in lung fibroblasts. (A) Associations of expression levels between FOXL1 and BMP ligands (BMP2 and BMP4) in lung fibroblast lines (n = 17) were evaluated by quantitative RT-PCR. The expression levels were normalized to that of GAPDH. (B) Cell numbers of NHLF transfected with siNC or siFOXL1 (#1 or #2) in the presence or absence of BMP4 (20 ng/ml) were counted 2, 4, and 6 days after seeding. The experiments were performed in triplicate. Error bars represent SEs. The cells transfected with siFOXL1 (#1 or #2) were compared with the siNC-transfected cells in the absence of BMP4. *P < 0.05. (C) NHLF transfected with siNC or siFOXL1 (#1 or #2) in the presence or absence of BMP4 (20 ng/ml) were embedded in collagen gels (n = 6). After 72 hours, the area of each gel was quantified. The percentage of contracted gel area was measured and compared with the initial size. Error bars represent SEs. The cells transfected with siFOXL1 (#1 or #2) were compared with the siNC-transfected cells in the absence of BMP4. *P < 0.05. n.s. = not significant.