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. 2020 Dec;63(6):831–842. doi: 10.1165/rcmb.2019-0396OC

Figure 6.

Figure 6.

FOXL1 is related to PDGF (platelet-derived growth factor) signaling in lung fibroblasts. (A) IB for PDGFRα (PDGF receptor-α) in NHLF and HFL1 treated with siNC or siFOXL1 (#1 or #2). α-tubulin was used as the loading control. (B) Cell numbers of NHLF or HFL1 (human fetal lung fibroblasts) transfected with siNC or siFOXL1 (#1 or #2) in the presence or absence of PDGF-AA (10 ng/ml) were counted 2, 4, and 6 days after seeding. The experiments were performed in quadruplicate. Error bars represent SEs. The cells transfected with siFOXL1 (#1 or #2) were compared with the siNC-transfected cells in the absence of PDGF-AA. *P < 0.05. (C) NHLF or HFL1 transfected with siNC or siFOXL1 (#1 or #2) in the presence or absence of PDGF-AA (10 ng/ml) were embedded in collagen gels. After 72 hours, the area of each gel was quantified. Percentage of contracted gel area was measured and compared with the initial size. The experiments were performed in triplicate. Error bars represent SEs. The cells transfected with siFOXL1 (#1 or #2) were compared with the siNC-transfected cells in the absence of PDGF-AA. *P < 0.05.