Figure 3.

FKBP13 expression is induced by bleomycin (Bleo) in mice. Wild-type (WT) C57BL/6 mice and FKBP13^−/− mice (n = 4/group) were treated with Bleo (0.06 U/mouse) and assessed after 7 and 21 days. Lung tissue was stained for FKBP13 by IHC and IF. (A) Representative FKBP13 immunostaining images. Images were acquired using the automated Olympus VS120 slide scanner, which utilizes a stitching algorithm to reconstruct the whole specimen from overlapping image tiles. Magnified images demonstrating FKBP13-positive cells are shown in the inset. (B) Quantification of FKBP13%-stained area in WT mice by HALO image-analysis software. No FKBP13 staining was detected in FKBP13^−/− lungs. *P < 0.05 versus untreated mice. (C) Dual immunofluorescence analysis confirmed the colocalization of FKBP13 (red) with the epithelial-cell marker E-cadherin (E-Cad; green) in WT mice treated with Bleo. DAPI was used as a nuclear marker. (D) Percentage of FKBP13-positive area colocalizing with E-Cad. Scale bars, 100 μm. **P < 0.01 and ***P < 0.001 versus untreated mice. ^#P < 0.05 and ^###P < 0.001 versus Day 7. IF = immunofluorescence.