Figure 7.

FKBP13^−/− mice have impaired resolution of Bleo-induced pulmonary fibrosis. WT and FKBP13^−/− mice (n = 4–6/group) were treated with high-dose Bleo and assessed for changes in lung function and fibrosis at Day 50. (A and B) PV loops as assessed by using flexiVent and derivation of elastance. (C and D) Masson’s Trichrome staining of lung tissues for assessment of fibrosis and quantification of Trichrome-positive area by using HALO. Images were acquired using the automated Olympus VS120 slide scanner, which utilizes a stitching algorithm to reconstruct the whole specimen from overlapping image tiles. Scale bars, 100 μm. **P < 0.01 versus untreated mice. ^#P < 0.05 and ^##P < 0.01 between genotypes.