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. 2021 Apr 1;14:19. doi: 10.1186/s13072-021-00393-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — H3K36me2&3 are associated with transcriptional regulation in stage II gametocytes. a Average transcript abundance (log₂Cy5/Cy3) on days 0–7 of gametocyte (GC) development [36] for genes with H3K36me2&3 enrichment in coding regions (CDR, blue), 1.5 kb upstream of TSSs (TSS, red) or both (gold) with ribbons representing ± S.D. They grey shadowed region highlights days on which H3K36me2&3-associated transcriptional changes are most prominent. b The 420 H3K36me2&3-enriched and repressed genes were grouped into those associated with proliferation (178 genes, green) that are significantly associated (P < 0 .05) with the biological processes indicated. The number of enriched genes is represented as a % of all background genes associated with the biological processes. Legend: ar. = arrest; nucl. = nucleosome; assemb. = assembly; replic. = replication; mod. = modification; var. = variation. Full descriptions of gene names and their PlasmoDB IDs are available in Additional file 2: Table S4