Fig. 1.

Import receptors are essential and affect mitochondrial proteins differentially. (A and B) Growth curves of uninduced and induced ATOM46 3′-UTR RNAi and the ATOM69 ORF RNAi cell lines. (Inset) Immunoblots probed for ATOM46 and ATOM69, respectively. Elongation factor 1a (EF1a) serves as loading control. (C) The two RNAi cell lines were analyzed by SILAC combined with quantitative proteomics. Scatter plot depicting the change in abundance of 856 mitochondrial proteins after ablation of ATOM69 (x-axis) and ATOM46 (y-axis), respectively. Both targets of RNAi are efficiently down-regulated (open circles).