Fig. 2.

Monocyte recruitment and replenishment of peritoneal macrophage pools in mice with induced endometriosis. (A) LpM (F4/80^hi, MHCII^lo) and SpM (F4/80^lo, MHCII^hi) populations in the peritoneal fluid of mice. (B) Quantification of LpM and SpM of sham (n = 6 to 8 at each time point) and endometriosis mice at 1 (n = 6), 2 (n = 8), and 3 wk (n = 16) after endometrial tissue injection compared to naïve mice (n = 12). (C) Flow plot indicating gating of monocytes (F4/80^lo, Ly6C^hi) in peritoneal lavage fluid. (D) Quantification of monocyte numbers of sham and endometriosis mice at 1, 2, and 3 wk after tissue injection. (E) Flow plot demonstrating expression of Ccr2 on F4/80^hi macrophages in peritoneal lavage fluid from sham vs. endometriosis mice (2 wk after tissue injection). (F) Quantification of CCR2+, F4/80^hi cells from endometriosis mice (n = 5) compared to sham (n = 4) and naïve (n = 4) mice. Data are presented as mean ± SEM. Statistics were determined using a one-way ANOVA and a Tukey post hoc test. *P < 0.05, **P < 0.01.