Fig. 5.

More lesions are evident in Ccl2^−/− mice. Wild-type donor endometrium was generated as previously shown (Fig. 3A) and injected i.p. into ovariectomized Ccl2^−/− recipients as in the previous figure. Wild-type recipients were also used as controls. Lesions were recovered 2 wk after tissue injection. (A) LpM and SpM populations in peritoneal lavage fluid from wild-type and Ccl2^−/− mice with induced endometriosis. (B–D) Quantification of (B) LpM, (C) SpM, and (D) monocytes in peritoneal lavage fluid from wild-type (n = 5) and Ccl2^−/− (n = 5) mice with induced endometriosis. (E) Number of lesions recovered from wild-type and Ccl2^−/− mice. (F) Size of lesions recovered from wild-type (n = 6) and Ccl2/- (n = 7) mice with induced endometriosis (from two independent experiments). (G) Dual immunodetection for Ly6C (green) and F4/80 (red) on lesions recovered from wild-type and Ccl2^−/− mice. (H) Quantification of monocytes (Ly6C+) and monocyte-derived macrophages (Ly6C+, F4/80+) in lesions recovered from wild-type and Ccl2^−/− mice. Data are presented as mean ± SEM or 95% confidence intervals (E). Statistical significance was determined using a Student’s t test or Mann–Whitney U test. *P < 0.05.