Fig. 6.
A function-blocking Ccr2 mAb reduces monocyte numbers without significant impact on lesion number. (A) Schematic showing the experimental design; mice with induced endometriosis were treated with a control IgG (MC67; n = 9) or a function-blocking CCR2 mAb (MC21; n = 10, from two independent experiments) 6 h prior to endometrial tissue injection and daily for an additional 4 d. Lesions were recovered 5 d after tissue injection. (B) Flow plot demonstrating the numbers of Ly6Chi monocytes in mice with induced endometriosis treated with MC67 or MC21. (C–E) Quantification of (C) LpM, (D) SpM, and (E) monocytes in the peritoneal lavage fluid of mice with induced endometriosis. (F) Number of lesions recovered from mice treated with MC67 or MC21. (G) Size of lesions recovered from mice treated with MC67 or MC21. Data are presented as mean ± SEM or 95% confidence intervals (F). Statistical significance was determined using a Student’s t test or a Mann–Whitney U test. *P < 0.05.