Fig. 2.
Overexpression of UVR8G101S results in strongly enhanced UV-B photomorphogenesis. (A) Immunoblot analysis of UVR8 and actin (loading control) protein levels in wild type (Ws), uvr8-7, uvr8-7/Pro35S:UVR8 (UVR8-OX), and three independent uvr8-7/Pro35S:UVR8G101S (UVR8G101S-OX #2, #4, and #7) lines. (B) Representative image of plate-grown seedlings grown under UV-B. (C) Anthocyanin concentration of seedlings depicted in B. Values of independent measurements (red bars), means, and SEM are shown (n = 3). (D) Representative images of seedlings grown in white light or white light supplemented with UV-B. (Scale bar, 5 mm.) (E) Quantification of hypocotyl length of seedlings depicted in D (n > 60). Shared letters indicate no statistically significant difference in the means (P > 0.05). (F) Rosette phenotype of representative plants grown for 56 d under short-day conditions in white light or white light supplemented with UV-B. The closeups show UV-B-grown UVR8G101S-OX plants. (Scale bar, 5 mm.) (G) Survival of seedlings grown for 7 d in white light (nonacclimated) or white light supplemented with UV-B (acclimated) then exposed for varying durations (0 to 4 h) to UV-B stress. Pictures were taken after a 7-d recovery period.