Fig. 4.

UVR8^G101S,W285A distorts a critical interaction loop at the dimer interface. (A) Ribbon and surface representations of the UVR8^G101S,W285A dimer. Highlighted in magenta is the critical loop containing the G101S mutation. (B) Superposition of UVR8^G101S,W285A (blue) with a wild-type UVR8 (PDB:4D9S, yellow) in ribbon representation. The site of mutation, residue 101, is represented in a ball-and-stick manner and colored by their atomic identity. Residue 285 is represented by sticks. (C) Zoomed-in view of the site of the W285A mutation. The site of mutation is represented in a ball-and-stick manner and the surrounding residues are shown as sticks. (D) Zoomed-in view of the loop containing the G101S mutation. The loop is represented with each ball corresponding to a C_α carbon to highlight the structural rearrangements of the loop. (E) Superposition of UVR8^{D96N,D107N,W285A} (green) with UVR8 represented as in B. (F) Zoomed-in view of the loop containing G101 represented as in D.