Comparative analysis of ILP transcript abundance in dsMet, dsKr-h1, dsEcR, and dsE74 RNAi female mosquitoes. (A) RNAi knockdowns of Met and Kr-h1 repressed ILP 2, 6, and 7 transcripts but activated the other five ILPs. (B) Knockdowns of EcR and E74 elevated ILP 2 and 6 transcripts, down-regulated ILP 4 and 5, while displaying no significant effect on the other ILPs. dsLuc (RNAi-luciferase) was used as control. Data represent three biological replicates with 30 individuals in each and are shown as mean ± SEM *P < 0.05, **P < 0.01, ***P < 0.001.