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. 2021 Feb 1;118(6):e2023470118. doi: 10.1073/pnas.2023470118

Fig. 3.

Fig. 3.

Met, Kr-h1, E74A, and E74B regulate the transcription of ilp genes. (A) Luciferase reporter assay after cotransfection of expression vectors pAc-Met-Myc and pAc-Taiman-Flag, along with the reporter construct and JH III, indicates that Met is an activator of ilp6 gene transcription. (B) Luciferase reporter assay after cotransfection of the expression vector pAc-Kr-h1-Myc and reporter constructs points out the activation role of Kr-h1 in the transcription of ilps 2 and 6. (C) Luciferase reporter assay shows that Kr-h1 is a repressor of the transcription of ilps 4 and 5. (D) Luciferase reporter assay after cotransfection of expression vectors pAc-E74A-Myc and reporter constructs indicates that E74A is a transcription activator of ilp4 and ilp5 genes. (E) Luciferase reporter assay after cotransfection of expression vectors pAc-E74B-Myc and reporter constructs indicates E74B as a repressor of ilps 2 and 6 transcription. Treatments with the empty expression vector and no input DNA and motif mutation served as controls. Data represent six replicates and are shown as mean ± SEM **P < 0.01, ***P < 0.001.