Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 1;118(6):e2023470118. doi: 10.1073/pnas.2023470118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 4. — CRISPR-Cas9-mediated gene tagging for the determination of hemolymph ILP levels. (A) Diagram indicates ILP HDR of ssODN donor bearing HA tag inserted into sequences encoding the last codon of B chain amino terminuses of each ilp gene to generate HA-tagged mosquito lines (ilp-HA). Then the eggs from HA-tagged females were used for second tagging. ssODN donor bearing FLAG tag was inserted into sequences encoding the first codon of A chain amino terminuses of each ilp gene to generate HA and FLAG double-tagged mosquito lines (ilp-HF). (B) Hemolymph levels of ILPs in response to JH signaling. Hemolymph ILP-HA/FLAG content (pg/μL) was determined using ELISA in tagged females after RNAi Met or RNAi Kr-h1 treatments and respective controls. (C) Hemolymph levels of ILPs in response to 20E signaling. Hemolymph ILP-HA/FLAG content (pg/μL) was determined using ELISA in tagged females after RNAi EcR or RNAi E74 treatments and respective controls. Data represent three biological replicates with five individuals in each and are shown as mean ± SEM *P < 0.05, **P < 0.01, ***P < 0.001.